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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has been observed in several tumors, which leads to chromosome instability, and therefore could lead to tumorigenesis. This protein was located to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a important purpose in endorsing cell-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, happen to be described. Two further splice variants have been described but detailed nucleotide sequence info just isn't still out there.
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Blocking peptides are peptides that bind precisely on the focus on antibody and block antibody binding. These peptide generally contains the epitope regarded via the antibody.
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This tutorial outlines more possible optimization of mobile and immunoassay parameters to be certain the absolute best final results are...
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This guideline outlines additional attainable optimization of cellular and immunoassay parameters to guarantee the very best success are...
Antibodies bound to the blocking peptide now not bind for the epitope about the focus on protein. By evaluating the staining through the blocked antibody as opposed to the antibody on your own, you can see which staining is specific.
Goal Information and facts Cyclin E belongs towards the highly conserved cyclin relatives, whose associates are characterized by a spectacular periodicity in protein abundance through the cell cycle. Cyclins purpose as regulators of CDK kinases. Different cyclins exhibit distinctive expression and degradation styles which add to the temporal coordination of each mitotic event. Cyclin E types a complex with and functions to be a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S transition. This protein accumulates with the here G1-S stage boundary and is degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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